![]() ![]() We would urge more rigorous routine characterization of stains and detection methodologies as a critical approach to systematically improving these critically important tools for quantitative proteomics. Unfortunately, many of these quantitative criteria are not routinely or consistently addressed by most of the studies published to date. This review evaluates protein detection methods in terms of their quantitative attributes, including limit of detection (i.e., sensitivity), linear dynamic range, inter-protein variability, capacity for spot detection after 2D gel electrophoresis, and compatibility with subsequent mass spectrometric analyses. The most desired characteristic in selecting one stain over another is sensitivity, but this is far from the only important parameter. ![]() Commonly used staining approaches involve colorimetric dyes such as Coomassie Brilliant Blue, fluorescent dyes including Sypro Ruby, newly developed reactive fluorophores, as well as a plethora of others. Proteomics research relies heavily on visualization methods for detection of proteins separated by polyacrylamide gel electrophoresis.
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